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human cervix epithelial adeno carcinoma hela cells  (ATCC)


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    ATCC human cervix epithelial adeno carcinoma hela cells
    Human Cervix Epithelial Adeno Carcinoma Hela Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+epithelial+cervix+carcinoma+hela+cells/HeLa/pm41251347-195-0-14
    Average 99 stars, based on 10447 article reviews
    human cervix epithelial adeno carcinoma hela cells - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: High-Performance Proteomics Using Nano-, Capillary-, and Microflow Chromatographic Separations.
    Article Snippet: .. Human epithelial cervix carcinoma HeLa cells (ATCC, CCL2) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Invitrogen), supplemented with 10% fetal bovine serum, 100 U/mL penicillin (Invitrogen), and 100 μg/ mL streptomycin (Invitrogen). ..

    Article Title: High-Performance Proteomics Using Nano‑, Capillary‑, and Microflow Chromatographic Separations
    Article Snippet: .. Human epithelial cervix carcinoma HeLa cells (ATCC, CCL-2) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Invitrogen), supplemented with 10% fetal bovine serum, 100 U/mL penicillin (Invitrogen), and 100 μg/mL streptomycin (Invitrogen). ..

    Article Title: Robust, reproducible and quantitative analysis of thousands of proteomes by micro-flow LC–MS/MS
    Article Snippet: .. Human epithelial cervix carcinoma HeLa cells (ATCC, CCL-2) were cultured in DMEM (Gibco, Invitrogen), supplemented with 10% fetal bovine serum, 100 U/mL penicillin (Invitrogen), 100 μg/mL streptomycin (Invitrogen), at 37 °C, in a humidified incubator with 5% CO2. .. Cells were harvested at ∼80% confluence by washing twice with PBS buffer and subsequently adding lysis buffer containing 8 M Urea, 80 mM Tris-HCl (pH = 7.6), 1 × EDTA free protease inhibitors (complete mini, Roche), and 1 × Phosphatase inhibitors (Sigma Aldrich) directly to the cell culture plate.

    Article Title: Robust, reproducible and quantitative analysis of thousands of proteomes by micro-flow LC-MS/MS.
    Article Snippet: .. Human epithelial cervix carcinoma HeLa cells (ATCC, CCL-2) were cultured in DMEM (Gibco, Invitrogen), supplemented with 10% fetal bovine serum, 100 U/mL penicillin (Invitrogen), 100 μg/mL streptomycin (Invitrogen), at 37 °C, in a humidified incubator with 5% CO2. .. Cells were harvested at ∼80% confluence by washing twice with PBS buffer and subsequently adding lysis buffer containing 8M Urea, 80 mM Tris-HCl (pH= 7.6), 1 × EDTA free protease inhibitors (complete mini, Roche), and 1 × Phosphatase inhibitors (Sigma Aldrich) directly to the cell culture plate.

    Modification:

    Article Title: High-Performance Proteomics Using Nano-, Capillary-, and Microflow Chromatographic Separations.
    Article Snippet: .. Human epithelial cervix carcinoma HeLa cells (ATCC, CCL2) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Invitrogen), supplemented with 10% fetal bovine serum, 100 U/mL penicillin (Invitrogen), and 100 μg/ mL streptomycin (Invitrogen). ..

    Article Title: High-Performance Proteomics Using Nano‑, Capillary‑, and Microflow Chromatographic Separations
    Article Snippet: .. Human epithelial cervix carcinoma HeLa cells (ATCC, CCL-2) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Invitrogen), supplemented with 10% fetal bovine serum, 100 U/mL penicillin (Invitrogen), and 100 μg/mL streptomycin (Invitrogen). ..

    Article Title: Alternative splicing induces cytoplasmic localization of RBFOX2 protein in calcific tendinopathy.
    Article Snippet: Background: Calcific tendinopathy (CT) is characterized by deposits of calcium, most commonly found in the shoulder tendons.. The exact cause and pathogenesis of CT are not fully understood.. This study analyzed the expression pattern of RNA-binding protein fox-1 homolog 2 (RBFOX2), a crucial splicing regulator in tissue differentiation.

    Multiple Displacement Amplification:

    Article Title: Multilayered proteomics reveals molecular switches dictating ligand-dependent EGFR trafficking.
    Article Snippet: .. Human epithelial cervix carcinoma HeLa cells, breast adenocarcinoma cells MDA-MB231, and lung adenocarcinoma A549 cells were purchased from ATCC. ..



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    Figure 6. MeV infection conditions human primary <t>epithelial</t> cells for S. Typhimurium cytosolic hyperproliferation and renders HeLa cells more resistant to S. flexneri intracellular growth. (A) Human primary epithelial cells from liver biopsies were maintained in culture for 6 days, infected or not with MeV (MOI 0.03) for 48 h and coinfected with S. Typhimurium during the last 6 h prior to gentamycin protection assay. CFU values obtained after 2 and 6 h of infection were used to derive a fold replication index for both culture conditions. Cells obtained from two donors were analyzed in independent experiments. (B) Human primary hepatocytes from one donor (#2) were prepared as in (A), immuno-stained for bacteria (red) and nuclei (blue) and analyzed by confocal microscopy. Representative images are shown. The histograms represent the mean of the number of bacteria per nucleus. (C) Western blot analysis of SQSTM1 and ACTB expression by human primary hepatocytes (PHH) from one donor (#1) after infection with MeV for 48 h and coinfection with S. Typhimurium during the last 6 h. Histograms represent the amount of SQSTM1 relative to ACTB and normalized to the uninfected control. (D) HeLa cell cultures were kept uninfected or infected with MeV (MOI 0.03) for 48 h prior to S. flexneri infection during the last 6 h and gentamycin protection assay. CFU values obtained after 2 and 6 h of infection were used to derive a fold replication index for both conditions. The histograms represent the mean ± SD of 3 independent experiments. **p < 0.01.
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    Figure 6. MeV infection conditions human primary <t>epithelial</t> cells for S. Typhimurium cytosolic hyperproliferation and renders HeLa cells more resistant to S. flexneri intracellular growth. (A) Human primary epithelial cells from liver biopsies were maintained in culture for 6 days, infected or not with MeV (MOI 0.03) for 48 h and coinfected with S. Typhimurium during the last 6 h prior to gentamycin protection assay. CFU values obtained after 2 and 6 h of infection were used to derive a fold replication index for both culture conditions. Cells obtained from two donors were analyzed in independent experiments. (B) Human primary hepatocytes from one donor (#2) were prepared as in (A), immuno-stained for bacteria (red) and nuclei (blue) and analyzed by confocal microscopy. Representative images are shown. The histograms represent the mean of the number of bacteria per nucleus. (C) Western blot analysis of SQSTM1 and ACTB expression by human primary hepatocytes (PHH) from one donor (#1) after infection with MeV for 48 h and coinfection with S. Typhimurium during the last 6 h. Histograms represent the amount of SQSTM1 relative to ACTB and normalized to the uninfected control. (D) HeLa cell cultures were kept uninfected or infected with MeV (MOI 0.03) for 48 h prior to S. flexneri infection during the last 6 h and gentamycin protection assay. CFU values obtained after 2 and 6 h of infection were used to derive a fold replication index for both conditions. The histograms represent the mean ± SD of 3 independent experiments. **p < 0.01.
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    Figure 6. MeV infection conditions human primary <t>epithelial</t> cells for S. Typhimurium cytosolic hyperproliferation and renders HeLa cells more resistant to S. flexneri intracellular growth. (A) Human primary epithelial cells from liver biopsies were maintained in culture for 6 days, infected or not with MeV (MOI 0.03) for 48 h and coinfected with S. Typhimurium during the last 6 h prior to gentamycin protection assay. CFU values obtained after 2 and 6 h of infection were used to derive a fold replication index for both culture conditions. Cells obtained from two donors were analyzed in independent experiments. (B) Human primary hepatocytes from one donor (#2) were prepared as in (A), immuno-stained for bacteria (red) and nuclei (blue) and analyzed by confocal microscopy. Representative images are shown. The histograms represent the mean of the number of bacteria per nucleus. (C) Western blot analysis of SQSTM1 and ACTB expression by human primary hepatocytes (PHH) from one donor (#1) after infection with MeV for 48 h and coinfection with S. Typhimurium during the last 6 h. Histograms represent the amount of SQSTM1 relative to ACTB and normalized to the uninfected control. (D) HeLa cell cultures were kept uninfected or infected with MeV (MOI 0.03) for 48 h prior to S. flexneri infection during the last 6 h and gentamycin protection assay. CFU values obtained after 2 and 6 h of infection were used to derive a fold replication index for both conditions. The histograms represent the mean ± SD of 3 independent experiments. **p < 0.01.
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    Figure 6. MeV infection conditions human primary <t>epithelial</t> cells for S. Typhimurium cytosolic hyperproliferation and renders HeLa cells more resistant to S. flexneri intracellular growth. (A) Human primary epithelial cells from liver biopsies were maintained in culture for 6 days, infected or not with MeV (MOI 0.03) for 48 h and coinfected with S. Typhimurium during the last 6 h prior to gentamycin protection assay. CFU values obtained after 2 and 6 h of infection were used to derive a fold replication index for both culture conditions. Cells obtained from two donors were analyzed in independent experiments. (B) Human primary hepatocytes from one donor (#2) were prepared as in (A), immuno-stained for bacteria (red) and nuclei (blue) and analyzed by confocal microscopy. Representative images are shown. The histograms represent the mean of the number of bacteria per nucleus. (C) Western blot analysis of SQSTM1 and ACTB expression by human primary hepatocytes (PHH) from one donor (#1) after infection with MeV for 48 h and coinfection with S. Typhimurium during the last 6 h. Histograms represent the amount of SQSTM1 relative to ACTB and normalized to the uninfected control. (D) HeLa cell cultures were kept uninfected or infected with MeV (MOI 0.03) for 48 h prior to S. flexneri infection during the last 6 h and gentamycin protection assay. CFU values obtained after 2 and 6 h of infection were used to derive a fold replication index for both conditions. The histograms represent the mean ± SD of 3 independent experiments. **p < 0.01.
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    Image Search Results


    Figure 6. MeV infection conditions human primary epithelial cells for S. Typhimurium cytosolic hyperproliferation and renders HeLa cells more resistant to S. flexneri intracellular growth. (A) Human primary epithelial cells from liver biopsies were maintained in culture for 6 days, infected or not with MeV (MOI 0.03) for 48 h and coinfected with S. Typhimurium during the last 6 h prior to gentamycin protection assay. CFU values obtained after 2 and 6 h of infection were used to derive a fold replication index for both culture conditions. Cells obtained from two donors were analyzed in independent experiments. (B) Human primary hepatocytes from one donor (#2) were prepared as in (A), immuno-stained for bacteria (red) and nuclei (blue) and analyzed by confocal microscopy. Representative images are shown. The histograms represent the mean of the number of bacteria per nucleus. (C) Western blot analysis of SQSTM1 and ACTB expression by human primary hepatocytes (PHH) from one donor (#1) after infection with MeV for 48 h and coinfection with S. Typhimurium during the last 6 h. Histograms represent the amount of SQSTM1 relative to ACTB and normalized to the uninfected control. (D) HeLa cell cultures were kept uninfected or infected with MeV (MOI 0.03) for 48 h prior to S. flexneri infection during the last 6 h and gentamycin protection assay. CFU values obtained after 2 and 6 h of infection were used to derive a fold replication index for both conditions. The histograms represent the mean ± SD of 3 independent experiments. **p < 0.01.

    Journal: Autophagy

    Article Title: Measles virus-imposed remodeling of the autophagy machinery determines the outcome of bacterial coinfection.

    doi: 10.1080/15548627.2022.2107309

    Figure Lengend Snippet: Figure 6. MeV infection conditions human primary epithelial cells for S. Typhimurium cytosolic hyperproliferation and renders HeLa cells more resistant to S. flexneri intracellular growth. (A) Human primary epithelial cells from liver biopsies were maintained in culture for 6 days, infected or not with MeV (MOI 0.03) for 48 h and coinfected with S. Typhimurium during the last 6 h prior to gentamycin protection assay. CFU values obtained after 2 and 6 h of infection were used to derive a fold replication index for both culture conditions. Cells obtained from two donors were analyzed in independent experiments. (B) Human primary hepatocytes from one donor (#2) were prepared as in (A), immuno-stained for bacteria (red) and nuclei (blue) and analyzed by confocal microscopy. Representative images are shown. The histograms represent the mean of the number of bacteria per nucleus. (C) Western blot analysis of SQSTM1 and ACTB expression by human primary hepatocytes (PHH) from one donor (#1) after infection with MeV for 48 h and coinfection with S. Typhimurium during the last 6 h. Histograms represent the amount of SQSTM1 relative to ACTB and normalized to the uninfected control. (D) HeLa cell cultures were kept uninfected or infected with MeV (MOI 0.03) for 48 h prior to S. flexneri infection during the last 6 h and gentamycin protection assay. CFU values obtained after 2 and 6 h of infection were used to derive a fold replication index for both conditions. The histograms represent the mean ± SD of 3 independent experiments. **p < 0.01.

    Article Snippet: Cell lines, antibodies, and reagents Human cervix carcinoma epithelial cells (HeLa; ATCC, CRM-CCL-2) and HeLa cells stably expressing the GFPLC3 reporter construct were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 61,965,026) complemented with 10% fetal bovine serum (FBS), 50 μg/mL gentamicin.

    Techniques: Infection, Staining, Bacteria, Confocal Microscopy, Western Blot, Expressing, Control